Expressing:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Gene Expression:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Activation Assay:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Inhibition:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Quantitative RT-PCR:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Isolation:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Bacteria:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Incubation:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Lysis:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
RNA Extraction:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Reverse Transcription:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Infection:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Electron Microscopy:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Transmission Assay:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Permeability:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
Concentration Assay:
Article Title: Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS SV-HUC-1 human urothelial cells (CRL-9520, ATCC) were obtained from the American Type Culture Collection (Manassas, Virginia, VA, USA).. The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.The cells were cultured in F-12 K nutrient mixture medium (21127-022, GibcoTM, Thermo Fisher Scientific, Waltham, Massachusetts, MA, USA) supplemented with 10% fetal bovine serum (GibcoTM) and 1% penicillin–streptomycin (GibcoTM), respectively, in a humidified incubator with an atmosphere of 5% CO2 at 37 oC.
Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection
Article Snippet: We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.We therefore asked whether the defect observed for AR4 mutants in vivo stems from an inability to withstand acidification inside the host cell.. To elucidate the contribution of AR4 inside the urothelial cells, we turned to the human urothelial tissue culture model, using the ATCC 5637 (HTB-9) bladder cell line.. In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).In this assay, approximately 1% of cells typically become internalized to seed intracellular infection ( ).
Article Title: Hypoxia impairs urothelial barrier function by inhibiting the expression of tight junction proteins in SV‐HUC‐1 cells
Article Snippet: SV‐40 immortalized human urothelial cells (SV‐HUC‐1) used in this study were purchased from ATCC.. Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.Cells were cultured in medium (high‐glucose DMEM, gibco) mixed with 10% fetal bovine serum (CY101, Cellorlab) and 1% penicillin/streptomycin (HyClone) in humidified atmosphere of 21% O 2 , 5% CO 2 and 74% N 2 at 37°C.
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat
Article Snippet: For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2 .
Article Title: Interactive Effects of Copper-Doped Urological Implants with Tissue in the Urinary Tract for the Inhibition of Cell Adhesion and Encrustation in the Animal Model Rat.
Article Snippet: 2.2.1.. Biocompatibility of the Materials For urothelial cells, the biocompatibility of the samples was studied using human non-tumorigenic urothelial HUC-1 cells (ATCC, CRL-9520, LGC Standards GmbH, Wesel, Germany).. Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.Cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific) at 37 ◦C in a humidified atmosphere with 5% CO2.
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